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Biacore spr adsorption experiments
Response curves <t>(SPR</t> signal (RU) vs time for TGTG-A-GTGT on CH3–SAMs at 25° showing an irreversible <t>adsorption</t> process even for this small peptide. The large drop in the SPR response when pure buffer was introduced at 90 s is primarily due to the bulk-shift effect.
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Response curves (SPR signal (RU) vs time for TGTG-A-GTGT on CH3–SAMs at 25° showing an irreversible adsorption process even for this small peptide. The large drop in the SPR response when pure buffer was introduced at 90 s is primarily due to the bulk-shift effect.

Journal:

Article Title: Determination of the Adsorption Free Energy for Peptide-Surface Interactions by SPR Spectroscopy

doi: 10.1021/la8005772

Figure Lengend Snippet: Response curves (SPR signal (RU) vs time for TGTG-A-GTGT on CH3–SAMs at 25° showing an irreversible adsorption process even for this small peptide. The large drop in the SPR response when pure buffer was introduced at 90 s is primarily due to the bulk-shift effect.

Article Snippet: SPR Adsorption Experiments Adsorption experiments were conducted by SPR using a Biacore × instrument (Biacore, Inc., Piscataway, NJ).

Techniques: Adsorption